Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo

June 24, 2021 Leave a comment
Within the liver, the bile canaliculi of hepatocytes are linked to intrahepatic bile ducts lined with cholangiocytes, which take away cytotoxic bile from the liver tissue. Though liver organoids have been reported, it isn’t clear whether or not the purposeful connection between hepatocytes and cholangiocytes is recapitulated in these organoids. Right here, we report the technology of a hepatobiliary tubular organoid (HBTO) utilizing mouse hepatocyte progenitors and cholangiocytes.
Hepatocytes kind the bile canalicular community and secrete metabolites into the canaliculi, that are then transported into the biliary tubular construction. Hepatocytes in HBTO purchase and keep metabolic capabilities together with albumin secretion and cytochrome P450 actions, over the long run. On this research, we set up purposeful liver tissue incorporating a bile drainage system ex vivo. HBTO allow us to breed the transport of hepatocyte metabolites in liver tissue, and to analyze the best way through which the 2 varieties of epithelial cells set up purposeful connections.

A Mannequin of Experimental Steatosis In Vitro: Hepatocyte Cell Tradition in Lipid Overload-Conditioned Medium

Metabolic dysfunction-associated fatty liver illness (MAFLD), beforehand often called non-alcoholic fatty liver illness (NAFLD), is essentially the most prevalent liver illness worldwide resulting from its relationship with weight problems, diabetes sort 2, and dyslipidemia. Hepatic steatosis, the buildup of lipid droplets within the liver parenchyma, is a key characteristic of the illness previous the irritation noticed in steatohepatitis, fibrosis, and end-stage liver illness. Lipid accumulation in hepatocytes would possibly intrude with correct metabolism of xenobiotics and endogenous molecules, in addition to to induce mobile processes resulting in the advance of the illness. Though the experimental research of steatosis will be carried out in vivo, in vitro approaches to the research of steatosis are complementary instruments with totally different benefits.
Hepatocyte tradition in lipid overload-conditioned medium is a wonderful reproducible possibility for the research of hepatic steatosis permitting the identification of mobile processes associated to lipid accumulation, corresponding to oxidative and reticular stresses, autophagia, proliferation, cell demise, etcetera, in addition to different testing together with drug effectiveness, and toxicological testing, amongst many different potential functions. Right here, it was aimed to explain the methodology of hepatocyte cell tradition in lipid overload-conditioned medium.
Generation of functional liver organoids on combining hepatocytes and cholangiocytes with hepatobiliary connections ex vivo
HepG2 cells have been cultured in RMPI 1640 medium conditioned with sodium palmitate and sodium oleate. Importantly, the ratio of those two lipids is essential to favor lipid droplet accumulation, whereas sustaining cell proliferation and a average mortality price, as happens within the liver through the illness. The methodology, from the preparation of the lipid resolution shares, combination, addition to the medium, and hepatocyte tradition is proven. With this method, it’s potential to determine lipid droplets within the hepatocytes which might be readily observable by Oil-red O staining, in addition to curves of proliferation/mortality charges.

Single amino acid insertion permits purposeful transduction of murine hepatocytes with human liver tropic AAV capsids

Current successes in scientific gene remedy functions have intensified the curiosity in utilizing adeno-associated viruses (AAVs) as vectors for gene supply into human liver. An inherent intriguing attribute of AAVs is that vector variants range considerably of their means to transduce hepatocytes from totally different species.
This has traditionally restricted the worth of preclinical research utilizing rodent fashions for predicting the effectivity of AAV vectors in liver-targeted gene remedy scientific research. On this work, we aimed to analyze the important thing determinants of the noticed differential interspecies transduction skills amongst AAV variants. We took benefit of area swapping methods between AAV-KP1, a newly recognized variant with enhanced murine liver tropism, and AAV3b, which capabilities poorly in mice.
The systematic in vivo comparability of AAV3b/AAV-KP1 chimeric variants allowed us to determine a threonine insertion at place 265 inside variable area I (VR-I) as the important thing residue that confers murine hepatic transduction to human-derived clade B (AAV2-like) and clade C (AAV3b-like) variants. We suggest to make use of this insertion to generate phylogenetically associated AAV surrogates in assist of toxicology and dosing research within the murine liver mannequin.

Hepatocyte Development Issue and Major Systemic Amyloidosis

A 75-year-old-man skilled liver dysfunction and was recognized with decompensated liver cirrhosis. His serum hepatocyte development issue (HGF) was very excessive (16.24 ng/ml). As a result of the etiology was unclear, we thought-about the opportunity of amyloidosis. Biopsy of the mucosa of the abdomen, duodenum and rectum demonstrated amyloid deposition. From the findings of Congo purple staining and immunohistochemical analyses, we made a analysis of systemic amyloid light-chain amyloidosis. Sadly, the affected person died one month after the analysis. We thought-about that serum HGF was helpful for the analysis and prediction of prognosis of main systemic amyloidosis.

Characterization of a commercially accessible line of iPSC hepatocytes as fashions of hepatocyte perform and toxicity for regulatory functions

It has just lately turn out to be potential to provide hepatocytes from human induced pluripotent stem cells (iPSC-heps), which can supply some benefits over main human hepatocytes (parafina) within the regulatory setting. The goal of this analysis was to evaluate similarities and variations between commercially accessible iPSC-heps and Prim-heps in preliminary assays of drug metabolism, hepatotoxicity, and drug transport. Hepatocytes have been both cultured in collagen-coated 96-well plates (Prim-heps and Second-iPSC-heps) or in ultra-low adhesion plates as spheroids (3d-iPSC-heps). 3d-iPSC-heps have been used to boost physiological cell-cell contacts, which is important to keep up the phenotype of mature hepatocytes.
Cytochrome P450 (CYP) 3A4, CYP1A2, and CYP2B6 exercise ranges have been evaluated utilizing fluorescent assays. Section II metabolism was assessed by HPLC measurement of formation of glucuronides and sulfates of 4-methylumbelliferone, 1-naphthol, and estradiol. The toxicity of acetaminophen, amiodarone, aspirin, clozapine, tacrine, tamoxifen, and troglitazone was monitored utilizing a luminescent cell viability assay. Canaliculi formation was monitored by following the fluorescence of 5,6-carboxy-2′,7′-dichlorofluorescein diacetate. All tradition fashions confirmed comparable ranges of basal CYP3A4, CYP1A2 and CYP2B6 exercise.

Mouse Liver PrimaCell: Hepatocytes
2-82100 1 Kit Ask for price

Human Liver PrimaCell: Hepatocytes
2-96121 1 Kit Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium
9-25095 5 x 100 ml Ask for price

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat
4-20302 ea Ask for price

Human Hepatocytes
HC4230 500,000 Cells
EUR 991.2

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium
9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium
9-46121 5 x 100 ml Ask for price

Immortalized Rat Hepatocytes - SV40
T0078 1x106 cells / 1.0 ml Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-26095 1 Set Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat
4-20304 ea Ask for price

Human Hepatocytes - Opioid Addicted
HC4230OP 500000
EUR 1148.4

Human Hepatocytes - Alcohol Addicted
HC4230AA 500,000 Cells
EUR 1148.4

Immortalized Human Hepatocytes - Ras
T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40
T0101 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - hTERT
T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40
T0073 1x106 cells / 1.0 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-33100 1 Set Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-47121 1 Set Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80322 1 x 100 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t
T0074 1x106 cells / 1.0 ml Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t
T0071 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse
4-20301 ea Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)
T0099 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat
4-20306 ea Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80314 1 x 100 ml Ask for price

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat
4-20303 ea Ask for price

Rat Primary hepatic carcinoma ELISA kit
E02P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit
E02P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit
E02P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit
E01P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit
E01P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit
E01P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human primary hepatic carcinoma,PHC ELISA Kit
201-12-1673 96 tests
EUR 528
  • This primary hepatic carcinoma ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human primary hepatic carcinoma(PHC)ELISA Kit
GA-E1689HM-48T 48T
EUR 346.8

Human primary hepatic carcinoma(PHC)ELISA Kit
GA-E1689HM-96T 96T
EUR 559.2

Human primary hepatic carcinoma(PHC)ELISA Kit
QY-E03406 96T
EUR 433.2

Human primary hepatic carcinoma,PHC ELISA Kit
YLA2711HU-48T 48T
EUR 435

Human primary hepatic carcinoma,PHC ELISA Kit
YLA2711HU-96T 96T
EUR 562.5

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Hepatocyte growth factor (Hgf)
1-CSB-RP084844R
  • EUR 606
  • EUR 318
  • EUR 2192.4
  • EUR 919.2
  • EUR 1461.6
  • EUR 402
  • 100ug
  • 10ug
  • 1MG
  • 200ug
  • 500ug
  • 50ug
  • MW: 55 kDa
  • Buffer composition: Tris-based buffer with 50% glycerol.
Description: Recombinant Rat Hepatocyte growth factor(Hgf),partial expressed in E.coli

Rat Hepatocyte Growth Factor ELISA kit
E02H0208-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Hepatocyte Growth Factor ELISA kit
E02H0208-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Hepatocyte Growth Factor ELISA kit
E02H0208-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Hepatocyte Growth Factor (HGF) CLIA Kit
20-abx190709
  • EUR 10131.6
  • EUR 5396.4
  • EUR 1243.2
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Hepatocyte Growth Factor (HGF) CLIA Kit
abx195673-96tests 96 tests
EUR 990
  • Shipped within 5-12 working days.
Nonetheless, whereas Prim-heps confirmed a vigorous response to CYP inducing brokers, Second-iPSC-heps confirmed no response and 3d-iPSC-heps displayed an inconclusive response. Second-iPSC-heps confirmed diminished, but considerable, glucuronide and sulfate formation in comparison with Prim-heps. All tradition fashions confirmed comparable exercise in exams of hepatotoxicity, with Prim-heps usually being extra delicate. All fashions fashioned canaliculi able to transporting carboxy-2′,7′-dichlorofluorescein. The iPSC-heps seem like helpful for toxicity and transport research, however metabolic exercise will not be optimum, and metabolism research would profit from a extra mature mannequin.

Leave a Reply

Your email address will not be published.