Biliary excretion of excess iron in mice requires hepatocyte iron import by Slc39a14

August 18, 2021 Leave a comment
Iron is important for erythropoiesis and different organic processes, however is poisonous in extra. Dietary absorption of iron is a extremely regulated course of and is a serious determinant of physique iron ranges. Iron excretion, nonetheless, is taken into account a passive, unregulated course of, and the underlying pathways are unknown. Right here we investigated the function of metallic transporters SLC39A14 and SLC30A10 in biliary iron excretion.
Whereas SLC39A14 imports manganese into the liver and different organs beneath physiological circumstances, it imports iron beneath circumstances of iron extra. SLC30A10 exports manganese from hepatocytes into the bile. We hypothesized that biliary excretion of extra iron could be impaired by SLC39A14 and SLC30A10 deficiency.
We due to this fact analyzed biliary iron excretion in Slc39a14- and Slc30a10-deficient mice raised on iron-sufficient and -rich diets. Bile was collected surgically from mice then analyzed with non-heme iron assays, mass spectrometry, ELISAs, and an electrophoretic assay for iron-loaded ferritin. Our outcomes help a mannequin during which biliary excretion of extra iron requires iron import into hepatocytes by SLC39A14, adopted by iron export into bile predominantly as ferritin, with iron export occurring independently of SLC30A10. To our information, that is the primary report of a molecular determinant of mammalian iron excretion, and may function foundation for future investigations into mechanisms of iron excretion and relevance to iron homeostasis.
REN ELISA Kit| Rat Renin ELISA Kit
EF017245
Pir ELISA Kit| Rat Pirin ELISA Kit
EF019186
Kl ELISA Kit| Rat Klotho ELISA Kit
EF017474

Extracellular vesicles activate ATM-Chk2 signaling pathway by the intercellular switch of mitochondrial DNA in HBV-infected human hepatocytes

Hepatitis B virus (HBV) is a human hepatotropic pathogen inflicting hepatocellular carcinoma. We not too long ago obtained HBV-susceptible immortalized human hepatocyte NKNT-Three by exogenously expressing NTCP and its derived cell clones, #28.3.eight and #28.3.25.13 exhibiting totally different ranges of HBV susceptibility.
Within the current research, we confirmed that HBV an infection activated the ATM-Chk2 signaling pathway in #28.3.25.13 cells however not in #28.3.eight cells. Each the cell tradition supernatant and extracellular vesicles (EVs) derived from HBV-infected #28.3.25.13 cells additionally activated the ATM-Chk2 signaling pathway in naïve #28.3.25.13 cells.
Curiously, EVs derived from HBV-infected #28.3.25.13 cells included increased stage of mitochondrial DNA (mtDNA) than these from HBV-infected #28.3.eight cells. Primarily based on our outcomes, we suggest the novel mannequin that EVs mediate the activation of ATM-Chk2 signaling pathway by the intercellular switch of mtDNA in HBV-infected human hepatocyte.

Results of Iron Oxide Nanoparticles on Structural Group of Hepatocyte Chromatin: Grey Stage Co-occurrence Matrix Evaluation

Grey stage co-occurrence matrix (GLCM) evaluation is a up to date and progressive computer-based algorithm that can be utilized for the quantification of refined modifications in a mobile construction. On this work, we use this methodology for the detection of discrete alterations in hepatocyte chromatin distribution after in vivo publicity to iron oxide nanoparticles (IONPs). The research was carried out on 40 male, wholesome C57BL/6 mice divided into 4 teams: three experimental teams that acquired totally different doses of IONPs and 1 management group.
We describe the dose-dependent discount of chromatin textural uniformity measured as GLCM angular second second. Related modifications have been detected for chromatin textural uniformity expressed as the worth of inverse distinction second. To the perfect of our information, that is the primary research to research the affect of iron-based nanomaterials on hepatocyte GLCM parameters.
Additionally, that is the primary research to use discrete wavelet rework evaluation, as a supplementary methodology to GLCM, for the evaluation of hepatocyte chromatin construction in these circumstances. The outcomes could current the helpful foundation for future analysis on the applying of GLCM and DWT strategies in pathology and different medical analysis areas.

Selective Imaging of HClO within the Liver Tissue in vivo Utilizing a Close to-infrared Hepatocyte-specific Fluorescent Probe

Liver damage is typified by an inflammatory response. Hypochlorous acid (HClO), an vital endogenous reactive oxygen species, is considered a biomarker related to liver damage. Close to-infrared (NIR) fluorescent probes with the benefit of deep tissue penetrating and low auto-fluorescence interference are extra appropriate for bioimaging in vivo . Thus, on this work, we designed and synthesized a novel NIR hepatocyte-specific fluorescent probe named NHF .
Biliary excretion of excess iron in mice requires hepatocyte iron import by Slc39a14
The probe NHF confirmed quick response (< 3s), massive spectral variation, and good selectivity to hint HClO in buffer answer. By using N -acetylgalactosamine (parafina) because the concentrating on ligand, probe NHF could be actively delivered to the liver tissue of zebrafish and mice. It’s important that probe NHF is the primary NIR hepatocyte-specific fluorescent probe, which efficiently visualized the up-regulation of endogenous HClO within the oxygen-glucose deprivation/reperfusion (OGD/R) mannequin HepG2 cells and dynamically monitored APAP-induced endogenous HClO within the liver tissue of zebrafish and mice.

Potential protecting impact of hesperidin on hypoxia/reoxygenation-induced hepatocyte damage

Hesperidin (HDN) has been reported to have hydrogen radical- and hydrogen peroxide-removal actions and to serve an antioxidant function in organic techniques. Nonetheless, whether or not HDN protects hepatocytes (HCs) towards hypoxia/reoxygenation (H/R)-induced damage stays unknown. The current research aimed to discover the function of HDN in H/R-induced damage. HCs have been remoted and cultured beneath H/R circumstances with or with out HDN therapy.
HC harm was markedly induced beneath H/R, as indicated by cell viability, supernatant lactate dehydrogenase ranges and alanine aminotransferase ranges; nonetheless, HDN therapy considerably reversed HC damage. Oxidative stress markers (malondialdehyde, superoxide dismutase, glutathioneand reactive oxygen species) have been elevated markedly throughout H/R in HCs; nonetheless, this impact was considerably attenuated after publicity to HDN. In contrast with these of the management group, the mRNA expression ranges of IL-6 and TNF-α in HCs and the concentrations of IL-6 and TNF-α within the supernatants elevated considerably following H/R, and HDN considerably ameliorated these results.

Human Liver PrimaCell: Normal Hepatocytes Growth Medium
9-46121 5 x 100 ml Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Medium
9-25095 5 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium
9-32100 5 x 100 ml Ask for price

Human Hepatocytes - Opioid Addicted
HC4230OP 500000
EUR 1148.4

Human Hepatocytes - Alcohol Addicted
HC4230AA 500,000 Cells
EUR 1148.4

Immortalized Human Hepatocytes - Ras
T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - hTERT
T0058 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat
4-20302 ea Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-47121 1 Set Ask for price

Immortalized Rat Hepatocytes - SV40
T0078 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40
T0101 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40
T0073 1x106 cells / 1.0 ml Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-26095 1 Set Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)
9-33100 1 Set Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80314 1 x 100 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t
T0074 1x106 cells / 1.0 ml Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t
T0071 1x106 cells / 1.0 ml Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse
4-20301 ea Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat
4-20304 ea Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)
T0099 1x106 cells / 1.0 ml Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80322 1 x 100 ml Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes
9-80306 1 x 100 ml Ask for price

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat
4-20306 ea Ask for price

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat
4-20303 ea Ask for price

Human Primary hepatic carcinoma ELISA kit
E01P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit
E01P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Primary hepatic carcinoma ELISA kit
E01P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human primary hepatic carcinoma,PHC ELISA Kit
201-12-1673 96 tests
EUR 528
  • This primary hepatic carcinoma ELISA kit is validated to work with samples from whole blood, serum, plasma and cell culture supernatant.
Description: A quantitative ELISA kit for measuring Human in samples from biological fluids.

Human primary hepatic carcinoma(PHC)ELISA Kit
GA-E1689HM-48T 48T
EUR 346.8

Human primary hepatic carcinoma(PHC)ELISA Kit
GA-E1689HM-96T 96T
EUR 559.2

Human primary hepatic carcinoma(PHC)ELISA Kit
QY-E03406 96T
EUR 433.2

Human primary hepatic carcinoma,PHC ELISA Kit
YLA2711HU-48T 48T
EUR 435

Human primary hepatic carcinoma,PHC ELISA Kit
YLA2711HU-96T 96T
EUR 562.5

Rat Primary hepatic carcinoma ELISA kit
E02P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit
E02P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rat Primary hepatic carcinoma ELISA kit
E02P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Dog Primary hepatic carcinoma ELISA kit
E08P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Canine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Pig Primary hepatic carcinoma ELISA kit
E07P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Porcine Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Goat Primary hepatic carcinoma ELISA kit
E06P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Goat Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Mouse Primary hepatic carcinoma ELISA kit
E03P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Mouse Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Rabbit Primary hepatic carcinoma ELISA kit
E04P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Rabbit Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Monkey Primary hepatic carcinoma ELISA kit
E09P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Monkey Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Guinea pig Primary hepatic carcinoma ELISA kit
E05P0656-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Guinea pig Primary hepatic carcinoma in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Primary Human Neurons
HNC001 300,000 Cyroperserved Cells
EUR 1467.6

rec Hepatocyte Growth Factor (human)
H-5596.0002 2.0µg
EUR 194.4

rec Hepatocyte Growth Factor (human)
H-5596.0010 10.0µg
EUR 457.2

Human Hepatocyte Growth Factor ELISA kit
E01H0208-192T 192 tests
EUR 1524
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
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Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hepatocyte Growth Factor ELISA kit
E01H0208-48 1 plate of 48 wells
EUR 624
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hepatocyte Growth Factor ELISA kit
E01H0208-96 1 plate of 96 wells
EUR 822
  • Kit contents: 1. MICROTITER PLATE * 1 2. ENZYME CONJUGATE*1 vial 3. STANDARD A*1 vial 4. STANDARD B*1 vial 5. STANDARD C*1 vial 6. STANDARD D*1 vial 7. STANDARD E*1 vial 8. STANDARD F*1 vial 9. SUBSTRATE A*1 vial 10. SUBSTRATE B*1 vial 11. STOP SOLUT
  • Show more
Description: A competitive ELISA for quantitative measurement of Human Hepatocyte Growth Factor in samples from blood, plasma, serum, cell culture supernatant and other biological fluids. This is a high quality ELISA kit developped for optimal performance with samples from the particular species.

Human Hepatocyte Growth Factor (HGF) CLIA Kit
20-abx190049
  • EUR 9493.2
  • EUR 5058
  • EUR 1167.6
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Human Hepatocyte Growth Factor (HGF) CLIA Kit
abx195671-96tests 96 tests
EUR 990
  • Shipped within 5-12 working days.

Human Hepatocyte Growth Factor (HGF)CLIA Kit
SCA047Hu-10x96wellstestplate 10x96-wells test plate
EUR 6777.36
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Human Hepatocyte Growth Factor (HGF) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<12
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Description: This is Double-antibody Sandwich Chemiluminescent immunoassay for detection of Human Hepatocyte Growth Factor (HGF) in serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Western blotting demonstrated that microtubule-associated protein 1 gentle chain 3α (MAP1LC3A, also called LC3) and Beclin-1 protein expression ranges elevated, whereas sequestosome 1 ranges decreased throughout H/R following publicity to HDN. The variety of GFP-LC3 puncta in HCs following publicity to HDN was elevated in contrast with that noticed in HCs with out HDN publicity beneath the H/R circumstances after bafilomycin A1 therapy. In abstract, the current research demonstrated that HDN attenuated HC oxidative stress and inflammatory responses whereas enhancing autophagy throughout H/R. HDN could have a possible protecting impact on HCs throughout H/R-induced damage.
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