Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells

Ammonia has a cytotoxic impact and may due to this fact be used as a range agent for enrichment of zone I hepatocytes. Nevertheless, it has not but been decided whether or not ammonia-treated hepatocyte-like cells are capable of proliferate in vitro. On this research, we employed an ammonia choice technique to purify hepatocyte-like cells that had been differentiated from human embryonic stem cells (ESCs) and from induced pluripotent stem cells (iPSCs).
The resistance to cytotoxicity or cell loss of life by ammonia is probably going attributable to the metabolism of ammonia within the cells. Along with ammonia metabolism-related genes, ammonia-selected hepatocytes confirmed elevated expression of the cytochrome P450 genes. Moreover, the ammonia-selected cells achieved immortality or at the very least an equal life span to human pluripotent stem cells with out affecting expression of the liver-associated genes. Ammonia remedy together with in vitro propagation is helpful for acquiring massive portions of hepatocytes.

BAM15, a Mitochondrial Uncoupling Agent, Attenuates Irritation within the LPS Injection Mouse Mannequin: An Adjunctive Anti-Irritation on Macrophages and Hepatocytes

Controlof immune responses by means of the immunometabolism interference is attention-grabbing for sepsis remedy. Then, expression of immunometabolism-associated genes and BAM15, a mitochondrial uncoupling agent, was explored in a proinflammatory mannequin utilizing lipopolysaccharide (LPS) injection. Accordingly, the decreased expression of mitochondrial uncoupling proteins was demonstrated by transcriptomic evaluation on metabolism-associated genes in macrophages (RAW246.7) and by polymerase chain response in LPS-stimulated RAW246.7 and hepatocytes (Hepa 1-6).
Pretreatment with BAM15 at 24 h previous to LPS in macrophages attenuated supernatant inflammatory cytokines (IL-6, TNF-α, and IL-10), downregulated genes of proinflammatory M1 polarization (iNOS and IL-1β), upregulated anti-inflammatory M2 polarization (Arg1 and FIZZ), and decreased cell power standing (extracellular flux evaluation and ATP manufacturing). Likewise, BAM15 decreased expression of proinflammatory genes (IL-6, TNF-α, IL-10, and iNOS) and diminished cell power in hepatocytes. In LPS-administered mice, BAM15 attenuated serum cytokines, organ damage (liver enzymes and serum creatinine), and tissue cytokines (livers and kidneys), partially, by means of the improved phosphorylated αAMPK, a sensor of ATP depletion with anti-inflammatory property, within the liver, and diminished inflammatory monocytes/macrophages (Ly6C +ve, CD11b +ve) within the liver as detected by Western blot and circulate cytometry, respectively. In conclusion, a proof of idea for irritation attenuation of BAM15 by means of metabolic interference-induced anti-inflammation on macrophages and hepatocytes was demonstrated as a brand new technique of anti-inflammation in sepsis.

[Establishment and evaluation of hepatocyte injury model induced by LPS/D-galactosamine in vitro]

Goal To ascertain a novel hepatocyte damage mannequin induced by lipopolysaccharide/D-galactosamine (LPS/D-GalN) in vitro. Strategies Freshly remoted mouse main hepatocytes had been cultured in vitro and handled with totally different doses of tumor necrosis factor-α (TNF-α) and 5 mg/mL of D-GalN. The supernatants from hepatocyte tradition had been detected for alanine aminotransferase (ALT) exercise by chemiluminescence assay.
Bone marrow-derived macrophages (BMDMs) had been stimulated with 1 μg/mL of LPS and the extent of TNF-α in supernatants had been detected by ELISA. Main hepatocytes had been handled with the BMDM supernatants mixed with 5 mg/mL D-GalN or 50 ng/mL actinomycin D (ActD) for 24 hours. The extent of ALT from hepatocyte supernatant was detected and morphology of hepatocytes was noticed with microscopy.
 Ammonia-based enrichment and long-term propagation of zone I hepatocyte-like cells
BMDMs and hepatocytes had been co-cultured and handled with 1 μg/mL of LPS mixed with D-GalN or ActD for 24 hours. Hepatocyte damage was mirrored by the ALT exercise and hepatocyte morphology. Outcomes The ALT exercise was considerably elevated within the supernatants of hepatocytes handled with TNF-α and D-GalN, indicating the plain hepatocyte damage. Co-treatment with LPS-primed BMDM supernatants and D-GalN or ActD may trigger hepatocyte damage, as mirrored by markedly elevated ALT exercise and the deformed and cracked hepatocytes. Within the context of co-culture of BMDM and hepatocytes, remedy with LPS and D-GalN led to apparent hepatocyte damage as anticipated.
LPS mixed with ActD couldn’t trigger hepatocyte damage, because the BMDMs began to die sooner than they may secret TNF-α to destruct hepatocytes. Hepatocytes with regular morphology and deformed BMDMs had been noticed. Conclusion LPS/D-GalN can be utilized to induce hepatocyte damage in vitro. D-GalN, fairly than ActD, ought to be used as a transcriptional inhibitor when the TNF-α -induced hepatocyte damage is evaluated in a co-culture system of BMDMs and hepatocytes.

Lateral measurement of graphene oxide determines differential mobile uptake and cell loss of life pathways in Kupffer cells, LSECs, and hepatocytes

As a consultant two-dimensional (2D) nanomaterial, graphene oxide (GO) has proven excessive potential in lots of functions attributable to its massive floor space, excessive flexibility, and wonderful dispersibility in aqueous options. These properties make GO a super candidate for bio-imaging, drug supply, and most cancers remedy.
When delivered to the physique, GO has been proven to build up within the liver, the first accumulation web site of systemic supply or secondary unfold from different uptake websites, and induce liver toxicity. Nevertheless, the contribution of the GO physicochemical properties and particular person liver cell varieties to this toxicity is unclear attributable to property variations and various cell varieties within the liver.
Herein, we examine the results of GOs with small (GO-S) and huge (GO-L) lateral sizes in three main cell varieties in liver, Kupffer cells (KCs), liver sinusoidal endothelial cells (LSECs), and hepatocytes. Whereas GOs induced cytotoxicity in KCs, they induced considerably much less toxicity in LSECs and hepatocytes. For KCs, we discovered that GOs had been phagocytosed that triggered NADPH oxidase mediated plasma membrane lipid peroxidation, which results in PLC activation, calcium flux, mitochondrial ROS technology, and NLRP3 inflammasome activation. The following caspase-1 activation induced IL-1β manufacturing and GSDMD-mediated pyroptosis. These results had been lateral size-dependent with GO-L displaying stronger results than GO-S.

Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium

9-32100 5 x 100 ml Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Medium

9-46121 5 x 100 ml Ask for price

Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-26095 1 Set Ask for price

Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat

4-20302 ea Ask for price

Liver Dissociation System 4 (Hepatocytes, rat), Rat

4-20304 ea Ask for price

Rat Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80322 1 x 100 ml Ask for price

Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-33100 1 Set Ask for price

Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium)

9-47121 1 Set Ask for price

Liver Dissociation System 1 (Hepatocytes), Mouse

4-20301 ea Ask for price

Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80306 1 x 100 ml Ask for price

Human Liver Tissue Preparation Buffer: Normal Hepatocytes

9-80314 1 x 100 ml Ask for price

Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat

4-20306 ea Ask for price

Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat

4-20303 ea Ask for price

Immortalized Rat Hepatocytes - SV40

T0078 1x106 cells / 1.0 ml Ask for price

Human Hepatocytes

HC4230 500,000 Cells
EUR 826

Anti-Hepatocytes antibody

STJ16100176 100 µg
EUR 389

Human Hepatocytes - Alcohol Addicted

HC4230AA 500,000 Cells
EUR 957

Human Hepatocytes - Opioid Addicted

HC4230OP 500000
EUR 957

Immortalized Human Hepatocytes - hTERT

T0058 1x106 cells / 1.0 ml Ask for price

Immortalized Human Hepatocytes - Ras

T0059 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40

T0073 1x106 cells / 1.0 ml Ask for price

Immortalized Mouse Hepatocytes - SV40

T0101 1x106 cells / 1.0 ml Ask for price

Rat Bone PrimaCell: Normal Osteoblasts

2-82510 1 Kit Ask for price

Rat Heart PrimaCell 6: Cardiomyocytes

2-82593 1 Kit Ask for price

Immortalized Mouse Hepatocytes-Conditionally (ImHep)

T0099 1x106 cells / 1.0 ml Ask for price

Custom production of antibodies in 5 Rats using customer supplied antigen (std 63 days protocol)

RAT-5 1
EUR 1138

Rat Cartilage PrimaCell: Normal Articular Cartilage

2-82523 1 Kit Ask for price

Rat Pancreas PrimaCell: Normal Pancreatic Islets

2-82578 1 Kit Ask for price

Rat Bone Marrow PrimaCell: Hematopoietic Cells

2-82590 1 Kit Ask for price

Rat Glomerular PrimaCell: Glomerular Endothelial Cells

2-82592 1 Kit Ask for price

Rat Thyroid PrimaCell: Thyroid Epithelial Cells

2-82598 1 Kit Ask for price

Immortalized Hamster Hepatocytes-SV40 T+t

T0071 1x106 cells / 1.0 ml Ask for price

Immortalized Monkey Hepatocytes-SV40 T+t

T0074 1x106 cells / 1.0 ml Ask for price

Rat Liver Tissue Lysate

LYSATE0003 200ug
EUR 150
Description: This cell lysate is prepared from rat liver tissue using Boster's RIPA Lysis Buffer (AR0105) using a standard whole cell lysate protocol. The concentration was determined using the BCA assay process and then diluted using Dithiothreitol (DTT) and a reducing SDS sample loading buffer, heated for 5 minutes at 100˚C.

Rat Ferritin (Liver) Antibody

11901-05011 150 ug
EUR 217

Rat Bone PrimaCell: Normal Osteoblasts Growth Medium

9-25010 5 x 100 ml Ask for price

Rat Aorta PrimaCell: Normal Aortic Endothelial Cells

2-82504 1 Kit Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells

2-82509 1 Kit Ask for price

Rat Endothelium PrimaCell: Normal Vascular Endothelial Cells

2-82535 1 Kit Ask for price

Rat Placenta PrimaCell: Normal Placenta Amniotic Cells

2-82579 1 Kit Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells

2-82580 1 Kit Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells

2-82588 1 Kit Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells

2-82589 1 Kit Ask for price

Rat Kidney PrimaCell 6: Proximal Tubular Cells

2-82594 1 Kit Ask for price

Rat Pancreas PrimaCell 2: Pancreatic Epithelial Cells

2-82597 1 Kit Ask for price

Rat Phosphofructokinase, Liver (PFKL) Protein

20-abx167625
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-7 working days.

Rat Liver Whole tissue lysate

RAL-1464 1 mg
EUR 524

Rat Pancreas PrimaCell: Normal Pancreatic Islets Growth Medium

9-25078 5 x 100 ml Ask for price

Rat Heart PrimaCell 6: Normal Cardiomyocytes Growth Medium

9-25093 5 x 100 ml Ask for price

Rat Artery PrimaCell: Normal Coronary Artery Endothelial Cells

2-82505 1 Kit Ask for price

Rat Breast PrimaCell: Normal Mammary Epithelial Primary Cells

2-82522 1 Kit Ask for price

Rat Rectal PrimaCell: Normal Rectal Smooth Muscle Cells

2-82581 1 Kit Ask for price

Rat Stomach PrimaCell: Normal Stomach Mucosa Epithelial Cells

2-82585 1 Kit Ask for price

Rat Brain PrimaCell 10: Olfactory Bulb Ensheathing Cells

2-82591 1 Kit Ask for price

Rat Lung PrimaCell 7: Alveolar Epithelial Cells II

2-82596 1 Kit Ask for price

Mouse Bone PrimaCell: Normal Osteoblasts

2-82009 1 Kit Ask for price

Mouse Heart PrimaCell 6: Cardiomyocytes

2-82098 1 Kit Ask for price

Human Bone PrimaCell: Normal Osteoblasts

2-96012 1 Kit Ask for price

Human Heart PrimaCell 6: Cardiomyocytes

2-96119 1 Kit Ask for price

Rat Aorta PrimaCell: Normal Aortic Endothelial Cells Growth Medium

9-25004 5 x 100 ml Ask for price

Rat Bone Joint PrimaCell: Normal Synovial Cells Growth Medium

9-25009 5 x 100 ml Ask for price

Rat Endothelium PrimaCell: Normal Vascular Endothelial Cells Growth Medium

9-25035 5 x 100 ml Ask for price

Rat Placenta PrimaCell: Normal Placenta Amniotic Cells Growth Medium

9-25079 5 x 100 ml Ask for price

Rat Prostate PrimaCell: Normal Prostate Epithelial Cells Growth Medium

9-25080 5 x 100 ml Ask for price

Rat Ureter PrimaCell: Normal Ureter Epithelial Cells Growth Medium

9-25088 5 x 100 ml Ask for price

Rat Urethral PrimaCell: Normal Urethral Epithelial Cells Growth Medium

9-25089 5 x 100 ml Ask for price

Rat Bone Marrow PrimaCell: Normal Hematopoietic Cells Growth Medium

9-25090 5 x 100 ml Ask for price

Rat Glomerular PrimaCell: Normal Glomerular Endothelial Cells Growth Medium

9-25092 5 x 100 ml Ask for price

Rat Thyroid PrimaCell: Normal Thyroid Epithelial Cells Growth Medium

9-25098 5 x 100 ml Ask for price

Rat New Borns PrimaCell: Normal New Born Epidermal Keratinocytes

2-82575 1 Kit Ask for price

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

abx573958-96tests 96 tests
EUR 739
  • Shipped within 5-12 working days.

Rat FibrOut 2, for cartilage, liver

4-20529 1 ml Ask for price

Rat FibrOut 2, for cartilage, liver

4-20530 5 x 1 ml Ask for price

Rat Liver OptiTDS: Tissue Dissociation System

4-28322 1 Kit Ask for price

Rat Glycogen Phosphorylase, Liver (PYGL) Protein

20-abx066870
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Rat Glycogen Phosphorylase, Liver (PYGL) Protein

20-abx066872
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-15 working days.

Rat Glycogen Phosphorylase, Liver (PYGL) Protein

20-abx066873
  • EUR 704.00
  • EUR 286.00
  • EUR 2165.00
  • EUR 829.00
  • EUR 495.00
  • 100 ug
  • 10 ug
  • 1 mg
  • 200 ug
  • 50 ug
  • Shipped within 5-12 working days.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

20-abx155968
  • EUR 7237.00
  • EUR 3855.00
  • EUR 895.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Shipped within 5-7 working days.

Rat Ferritin (Liver) Antibody (Biotin Conjugate)

11901-05021 150 ug
EUR 276

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

RDR-PFKL-Ra-48Tests 48 Tests
EUR 583

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

RDR-PFKL-Ra-96Tests 96 Tests
EUR 811

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

RD-PFKL-Ra-48Tests 48 Tests
EUR 557

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

RD-PFKL-Ra-96Tests 96 Tests
EUR 775

Rat Phosphofructokinase, Liver ELISA Kit (PFKL)

RK03883 96 Tests
EUR 521

Rat Phosphofructokinase, Liver (PFKL) CLIA Kit

20-abx494255
  • EUR 8569.00
  • EUR 4560.00
  • EUR 1052.00
  • 10 × 96 tests
  • 5 × 96 tests
  • 96 tests
  • Please enquire.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

DLR-PFKL-Ra-48T 48T
EUR 549
  • Should the Rat Phosphofructokinase, Liver (PFKL) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Phosphofructokinase, Liver (PFKL) in samples from tissue homogenates, cell lysates or other biological fluids.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

DLR-PFKL-Ra-96T 96T
EUR 718
  • Should the Rat Phosphofructokinase, Liver (PFKL) ELISA Kit is proven to show malperformance, you will receive a refund or a free replacement.
Description: A sandwich quantitative ELISA assay kit for detection of Rat Phosphofructokinase, Liver (PFKL) in samples from tissue homogenates, cell lysates or other biological fluids.

Phosphofructokinase, Liver (PFKL) Polyclonal Antibody (Rat)

4-PAD406Ra01
  • EUR 259.00
  • EUR 2708.00
  • EUR 670.00
  • EUR 328.00
  • EUR 219.00
  • 100ul
  • 10ml
  • 1ml
  • 200ul
  • 20ul
  • Sequence of the immunogen: PFKL (Ala2~His390)
  • Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Description: A Rabbit polyclonal antibody against Rat Phosphofructokinase, Liver (PFKL)

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

SED406Ra-10x96wellstestplate 10x96-wells test plate
EUR 5124.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phosphofructokinase, Liver (PFKL) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<1
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phosphofructokinase, Liver (PFKL) in tissue homogenates, cell lysates and other biological fluids.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

SED406Ra-1x48wellstestplate 1x48-wells test plate
EUR 509.64
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phosphofructokinase, Liver (PFKL) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<1
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phosphofructokinase, Liver (PFKL) in tissue homogenates, cell lysates and other biological fluids.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

SED406Ra-1x96wellstestplate 1x96-wells test plate
EUR 685.2
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phosphofructokinase, Liver (PFKL) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<1
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phosphofructokinase, Liver (PFKL) in tissue homogenates, cell lysates and other biological fluids.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

SED406Ra-5x96wellstestplate 5x96-wells test plate
EUR 2783.4
  • The Intra-assay Precision is determined when 3 samples with low, middle and high level of Rat Phosphofructokinase, Liver (PFKL) were tested on 3 different plates, 8 replicates in each plate
  • CV(%) = SD/meanX100
  • Intra-Assay: CV<10%
  • Inter-Assay: CV<1
  • Show more
Description: This is Double-antibody Sandwich Enzyme-linked immunosorbent assay for detection of Rat Phosphofructokinase, Liver (PFKL) in tissue homogenates, cell lysates and other biological fluids.

Rat Phosphofructokinase, Liver (PFKL) ELISA Kit

4-SED406Ra
  • EUR 5175.00
  • EUR 2734.00
  • EUR 686.00
  • 10 plates of 96 wells
  • 5 plates of 96 wells
  • 1 plate of 96 wells
  • Known also as Phosphofructokinase, Liver elisa. Alternative names of the recognized antigen: PFK-B
  • 6-phosphofructokinase type B
  • Phosphofructo-1-kinase isozyme B
  • Phosphohexokinase
Description: Enzyme-linked immunosorbent assay based on the Double-antibody Sandwich method for detection of Rat Phosphofructokinase, Liver (PFKL) in samples from tissue homogenates, cell lysates and other biological fluids with no significant corss-reactivity with analogues from other species.

Rat liver ferritin purified, ELISA grade

FERT21-R-50 50 ug
EUR 408

Rabbit Anti-Rat liver Ferritin IgG

FERT25-A 100 ul
EUR 482

Chicken anti-Rat liver Ferritin IgG

FERT26-A 100 ul
EUR 482
Amongst the liver cell varieties, decreased cell affiliation and the absence of lipid peroxidation resulted in low cytotoxicity in LSECs and hepatocytes. Utilizing further GO samples with totally different lateral sizes, floor functionalities, or thickness, we additional confirmed the differential cytotoxic results in liver cells and the key position of GO lateral measurement in KUP5 pyroptosis by correlation research. These findings delineated the GO results on mobile uptake and cell loss of life pathways in liver cells, and supply useful data to additional consider GO results on the liver for biomedical functions.

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