Ammonia has a cytotoxic impact and may due to this fact be used as a range agent for enrichment of zone I hepatocytes. Nevertheless, it has not but been decided whether or not ammonia-treated hepatocyte-like cells are capable of proliferate in vitro. On this research, we employed an ammonia choice technique to purify hepatocyte-like cells that had been differentiated from human embryonic stem cells (ESCs) and from induced pluripotent stem cells (iPSCs).
The resistance to cytotoxicity or cell loss of life by ammonia is probably going attributable to the metabolism of ammonia within the cells. Along with ammonia metabolism-related genes, ammonia-selected hepatocytes confirmed elevated expression of the cytochrome P450 genes. Moreover, the ammonia-selected cells achieved immortality or at the very least an equal life span to human pluripotent stem cells with out affecting expression of the liver-associated genes. Ammonia remedy together with in vitro propagation is helpful for acquiring massive portions of hepatocytes.
BAM15, a Mitochondrial Uncoupling Agent, Attenuates Irritation within the LPS Injection Mouse Mannequin: An Adjunctive Anti-Irritation on Macrophages and Hepatocytes
Controlof immune responses by means of the immunometabolism interference is attention-grabbing for sepsis remedy. Then, expression of immunometabolism-associated genes and BAM15, a mitochondrial uncoupling agent, was explored in a proinflammatory mannequin utilizing lipopolysaccharide (LPS) injection. Accordingly, the decreased expression of mitochondrial uncoupling proteins was demonstrated by transcriptomic evaluation on metabolism-associated genes in macrophages (RAW246.7) and by polymerase chain response in LPS-stimulated RAW246.7 and hepatocytes (Hepa 1-6).
Pretreatment with BAM15 at 24 h previous to LPS in macrophages attenuated supernatant inflammatory cytokines (IL-6, TNF-α, and IL-10), downregulated genes of proinflammatory M1 polarization (iNOS and IL-1β), upregulated anti-inflammatory M2 polarization (Arg1 and FIZZ), and decreased cell power standing (extracellular flux evaluation and ATP manufacturing). Likewise, BAM15 decreased expression of proinflammatory genes (IL-6, TNF-α, IL-10, and iNOS) and diminished cell power in hepatocytes. In LPS-administered mice, BAM15 attenuated serum cytokines, organ damage (liver enzymes and serum creatinine), and tissue cytokines (livers and kidneys), partially, by means of the improved phosphorylated αAMPK, a sensor of ATP depletion with anti-inflammatory property, within the liver, and diminished inflammatory monocytes/macrophages (Ly6C +ve, CD11b +ve) within the liver as detected by Western blot and circulate cytometry, respectively. In conclusion, a proof of idea for irritation attenuation of BAM15 by means of metabolic interference-induced anti-inflammation on macrophages and hepatocytes was demonstrated as a brand new technique of anti-inflammation in sepsis.
[Establishment and evaluation of hepatocyte injury model induced by LPS/D-galactosamine in vitro]
Goal To ascertain a novel hepatocyte damage mannequin induced by lipopolysaccharide/D-galactosamine (LPS/D-GalN) in vitro. Strategies Freshly remoted mouse main hepatocytes had been cultured in vitro and handled with totally different doses of tumor necrosis factor-α (TNF-α) and 5 mg/mL of D-GalN. The supernatants from hepatocyte tradition had been detected for alanine aminotransferase (ALT) exercise by chemiluminescence assay.
Bone marrow-derived macrophages (BMDMs) had been stimulated with 1 μg/mL of LPS and the extent of TNF-α in supernatants had been detected by ELISA. Main hepatocytes had been handled with the BMDM supernatants mixed with 5 mg/mL D-GalN or 50 ng/mL actinomycin D (ActD) for 24 hours. The extent of ALT from hepatocyte supernatant was detected and morphology of hepatocytes was noticed with microscopy.
BMDMs and hepatocytes had been co-cultured and handled with 1 μg/mL of LPS mixed with D-GalN or ActD for 24 hours. Hepatocyte damage was mirrored by the ALT exercise and hepatocyte morphology. Outcomes The ALT exercise was considerably elevated within the supernatants of hepatocytes handled with TNF-α and D-GalN, indicating the plain hepatocyte damage. Co-treatment with LPS-primed BMDM supernatants and D-GalN or ActD may trigger hepatocyte damage, as mirrored by markedly elevated ALT exercise and the deformed and cracked hepatocytes. Within the context of co-culture of BMDM and hepatocytes, remedy with LPS and D-GalN led to apparent hepatocyte damage as anticipated.
LPS mixed with ActD couldn’t trigger hepatocyte damage, because the BMDMs began to die sooner than they may secret TNF-α to destruct hepatocytes. Hepatocytes with regular morphology and deformed BMDMs had been noticed. Conclusion LPS/D-GalN can be utilized to induce hepatocyte damage in vitro. D-GalN, fairly than ActD, ought to be used as a transcriptional inhibitor when the TNF-α -induced hepatocyte damage is evaluated in a co-culture system of BMDMs and hepatocytes.
Lateral measurement of graphene oxide determines differential mobile uptake and cell loss of life pathways in Kupffer cells, LSECs, and hepatocytes
As a consultant two-dimensional (2D) nanomaterial, graphene oxide (GO) has proven excessive potential in lots of functions attributable to its massive floor space, excessive flexibility, and wonderful dispersibility in aqueous options. These properties make GO a super candidate for bio-imaging, drug supply, and most cancers remedy.
When delivered to the physique, GO has been proven to build up within the liver, the first accumulation web site of systemic supply or secondary unfold from different uptake websites, and induce liver toxicity. Nevertheless, the contribution of the GO physicochemical properties and particular person liver cell varieties to this toxicity is unclear attributable to property variations and various cell varieties within the liver.
Herein, we examine the results of GOs with small (GO-S) and huge (GO-L) lateral sizes in three main cell varieties in liver, Kupffer cells (KCs), liver sinusoidal endothelial cells (LSECs), and hepatocytes. Whereas GOs induced cytotoxicity in KCs, they induced considerably much less toxicity in LSECs and hepatocytes. For KCs, we discovered that GOs had been phagocytosed that triggered NADPH oxidase mediated plasma membrane lipid peroxidation, which results in PLC activation, calcium flux, mitochondrial ROS technology, and NLRP3 inflammasome activation. The following caspase-1 activation induced IL-1β manufacturing and GSDMD-mediated pyroptosis. These results had been lateral size-dependent with GO-L displaying stronger results than GO-S.
Mouse Liver PrimaCell: Normal Hepatocytes Growth Medium |
9-32100 |
CHI Scientific |
5 x 100 ml |
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Human Liver PrimaCell: Normal Hepatocytes Growth Medium |
9-46121 |
CHI Scientific |
5 x 100 ml |
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Rat Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium) |
9-26095 |
CHI Scientific |
1 Set |
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Mouse Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium) |
9-33100 |
CHI Scientific |
1 Set |
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Human Liver PrimaCell: Normal Hepatocytes Growth Supplements with Serum (for 500 ml medium) |
9-47121 |
CHI Scientific |
1 Set |
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Rabbit Liver Hepatocytes |
ABC-TC4061 |
AcceGen |
1 vial |
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|
Description: Rabbit liver epithelial cells, 5-week rabbit |
Liver Dissociation System 2 (Hepatocytes, Parenchymal hepatocytes), Mouse and Rat |
4-20302 |
CHI Scientific |
ea |
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Liver Dissociation System 4 (Hepatocytes, rat), Rat |
4-20304 |
CHI Scientific |
ea |
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Rat Liver Tissue Preparation Buffer: Normal Hepatocytes |
9-80322 |
CHI Scientific |
1 x 100 ml |
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Liver Dissociation System 1 (Hepatocytes), Mouse |
4-20301 |
CHI Scientific |
ea |
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Liver Dissociation System 6 (Hepatocytes,Nonparenchymal), Mouse and Rat |
4-20306 |
CHI Scientific |
ea |
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Mouse Liver Tissue Preparation Buffer: Normal Hepatocytes |
9-80306 |
CHI Scientific |
1 x 100 ml |
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Human Liver Tissue Preparation Buffer: Normal Hepatocytes |
9-80314 |
CHI Scientific |
1 x 100 ml |
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Primary Rat (Fischer) Hepatocytes, Plateable |
T0108 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Rat (Fischer) Hepatocytes, Suspension |
T0107 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Liver Dissociation System 3 (Hepatocytes, Kupffer, Parenchymal), Mouse and Rat |
4-20303 |
CHI Scientific |
ea |
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Primary Rat (Wistar Han) Hepatocytes, Plateable |
T0106 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Rat (Wistar Han) Hepatocytes, Suspension |
T0105 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Rat (Sprague Dawley) Hepatocytes, Plateable |
T0104 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Rat (Sprague Dawley) Hepatocytes, Suspension |
T0103 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Rat Hepatocytes |
ABC-TC4136 |
AcceGen |
1 vial |
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|
Description: Rat Hepatocytes (RH) are isolated from adult rat livers. They are cryopreserved at the end of primary cell isolation. |
Primary Dog (Beagle) Hepatocytes, Plateable |
T0118 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Dog (Beagle) Hepatocytes, Suspension |
T0117 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Normal Human Primary Plateable Hepatocytes |
T5539 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 925 |
Normal Human Primary Plateable Hepatocytes |
T5541 |
ABM |
1x10^6 cells / 1.0 ml |
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Normal Human Primary Suspension Hepatocytes |
T5538 |
ABM |
1x10^6 cells / 1.0 ml |
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Primary Mouse (CD-1) Hepatocytes, Plateable |
T0120 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Human Plateable Hepatocytes From Whole Livers |
ABC-TC4022 |
AcceGen |
1 vial |
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|
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. |
Primary Mouse (CD-1) Hepatocytes, Suspension |
T0119 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Mouse (C57BI/6) Hepatocytes, Plateable |
T0122 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Mouse (C57BI/6) Hepatocytes, Suspension |
T0121 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Enhanced Primary Human Hepatocytes - 5 million |
T5996 |
ABM |
5x10^6 cells / 1.0 ml |
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Primary Monkey (Cynomolgus) hepatocytes, Plateable |
T0112 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Minipig (Gottingen) Hepatocytes, Suspension |
T0113 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Monkey (Cynomolgus) Hepatocytes, Suspension |
T0111 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Enhanced Primary Human Hepatocytes – 1 Million |
T5995 |
ABM |
1x10^6 cells / 1.0ml |
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Rat Plateable Hepatocytes |
ABC-TC4024 |
AcceGen |
1 vial |
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|
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. |
Primary Human Hepatocytes, Transporter Uptake Qualified |
T0125 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 3500 |
Primary Human Hepatocytes, Induction Qualified, Plateable |
T0128 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 2625 |
Primary Human Hepatocytes, Metabolism Qualified, Plateable |
T0124 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 2625 |
Primary Rabbit (New Zealand White) Hepatocytes, Suspension |
T0115 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Primary Human Hepatocytes, Metabolism Qualified, Suspension |
T0123 |
ABM |
5x10^5 cells / 1.0 ml |
EUR 1375 |
Immortalized Rat Hepatocytes - SV40 |
T0078 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
Rat Hepatocytes Suspension-Adult |
ABC-TC4137 |
AcceGen |
1 vial |
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|
Description: Rat Hepatocytes Suspension - Adult |
Human Hepatocytes |
ABC-TC3646 |
AcceGen |
1 vial |
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|
Description: The liver fulfills many vital processes in mammals. It is the central organ of energy metabolism, biotransformation of xenobiotics, and synthesis of plasma proteins under physiological and pathophysiological conditions. Primary human hepatocyte culture is an important experimental model for the study of liver specific function. Propagation of human hepatocytes is critical for gene therapy, cell transplantation and clinical liver support systems. In appropriate culture condition, cultured human hepatocytes proliferate and maintain differentiated hepatocyte function such as the synthesis of serum proteins. HH from Gentaur Research Laboratories are isolated from human liver. HH are cryopreserved immediately after purification and delivered frozen. Each vial contains 1 x 10^6 cells in 1 ml volume. HH are characterized by immunofluorescent method with antibodies to albumin, cytokeratin-18 and vimentin. HH are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. HH is not recommended for expanding or long term cultures since the cells do not proliferate in culture. |
Mouse Hepatocytes |
ABC-TC3928 |
AcceGen |
1 vial |
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|
Description: Our High Quality Mouse Hepatocytes have been isolated from mouse livers after liver perfusion and low-speed differential centrifugation. The hepatocytes are cryoperserved immediately after the experimental process and delivered frozen. |
Human Hepatocytes |
HC4230 |
Neuromics |
500,000 Cells |
EUR 991.2 |
Bovine Hepatocytes |
ABC-TC3132 |
AcceGen |
1 vial |
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|
Description: Bovine hepatocytes, 4-week bovine |
Cryo Rat (Wistar) Hepatocytes, Plateable |
LORICP01 |
Westburg |
each |
EUR 552.24 |
Cryo Rat (Wistar) Hepatocytes, Suspension |
LORICS01 |
Westburg |
each |
EUR 328.97 |
Cryo Rat (Sprague Dawley) Hepatocytes, Plateable |
LORSCP01 |
Westburg |
each |
EUR 552.24 |
Conditionally Immortalized Rat Hepatocytes-SV40T |
CSC-I2094Z |
Creative Bioarray |
One Frozen vial |
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Cryo Rat (Sprague Dawley) Hepatocytes, Suspension |
LORSCS01 |
Westburg |
each |
EUR 328.97 |
Cryo Rat (Wistar Hannover) Hepatocytes, Plateable |
LORWCP01 |
Westburg |
each |
EUR 552.24 |
Plateable Mouse Hepatocytes |
ABC-TC4023 |
AcceGen |
1 vial |
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|
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. |
Cryo Rat (Wistar Hannover) Hepatocytes, Suspension |
LORWCS01 |
Westburg |
each |
EUR 328.97 |
Wistar male rat HEPATOCYTES , 10 million cells |
LORIFS10-EU |
Westburg |
each |
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Human Cancer PrimaCell10: Liver Tumor Cells |
2-96033 |
CHI Scientific |
1 Kit |
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Immortalized human hepatocytes |
ABI-TC3500 |
AcceGen |
1 vial |
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Description: The cell line was prepared by immortalizing hepatocytes from a 12 year-old Caucasian female donor with the SV40 large T antigen. The donor tested negative for CMV, HIV, HBV, and HCV. |
Immortalized Monkey Hepatocytes |
CSC-I2072Z |
Creative Bioarray |
One Frozen vial |
Ask for price |
Plateable DOG (Beagle) Hepatocytes |
ABC-TC4021 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. |
Human iPSC-derived Hepatocytes |
CSC-00863L |
Creative Bioarray |
One Frozen vial |
Ask for price |
|
Human Hepatocytes - Opioid Addicted |
HC4230OP |
Neuromics |
500000 |
EUR 1148.4 |
Human Hepatocytes - Opioid Addicted |
HC4230OP-B |
Neuromics |
each |
EUR 805.5 |
Human Fresh Hepatocytes, Suspension |
ABC-TC3432 |
AcceGen |
1 vial |
Ask for price |
|
Description: Freshly isolated human hepatocytes represent a living biological system in which to measure metabolism, induction, toxicity, and transporter studies. |
Human Hepatocytes - Alcohol Addicted |
HC4230AA |
Neuromics |
500,000 Cells |
EUR 1148.4 |
Human Hepatocytes - Alcohol Addicted |
HC4230AA-B |
Neuromics |
each |
EUR 625.5 |
Immortalized Human Hepatocytes - Ras |
T0059 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Human Pooled Hepatocytes(Heps 10) |
ABC-TC3458 |
AcceGen |
1 vial |
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|
Description: Human Pooled Primary Hepatocytes (Heps) provide an ideal culture model for drug testing and drug development.Heps 20 Human Pooled Primary Hepatocytes contain cryopreserved hepatocytes pooled from 20 different donors and have undergone only one freeze cycle. Pooled hepatocytes provide the genetic diversity necessary for superior reliability and predictability of the metabolic characteristics of a test compound relative to a representative population. Pooled hepatocytes increase experimental control and reduce costs by eliminating the need to conduct redundant experiments necessary to obtain population-based data. Each lot has undergone a single freeze cycle, assuring maximum hepatocyte viability and consistency from donor to donor within the pool.Cell Features:Each donor's hepatocytes have been cryopreserved ONCE immediately after isolation and purification.Pools of 10 or 20 donors are available. Each donor is represented by a statistically significant number of cells relative to the pool.There is extensive quality control providing characterization of viability and enzyme activity from both individual donors and the total pool.Gentaur guarantees performance and quality.Donor information is available. |
Human Pooled Hepatocytes(Heps 20) |
ABC-TC3459 |
AcceGen |
1 vial |
Ask for price |
|
Description: Human Pooled Primary Hepatocytes (HP2 Heps) provide an ideal culture model for drug testing and drug development. HP2 10 Human Pooled Primary Hepatocytes contain cryopreserved hepatocytes pooled from 10 different donors and have undergone only one freeze cycle. Pooled hepatocytes provide the genetic diversity necessary for superior reliability and predictability of the metabolic characteristics of a test compound relative to a representative population. Pooled hepatocytes increase experimental control and reduce costs by eliminating the need to conduct redundant experiments necessary to obtain population-based data. Each lot has undergone a single freeze cycle, assuring maximum hepatocyte viability and consistency from donor to donor within the pool.Cell Features:Each donor's hepatocytes have been cryopreserved ONCE immediately after isolation and purification.Pools of 10 or 20 donors are available. Each donor is represented by a statistically significant number of cells relative to the pool.There is extensive quality control providing characterization of viability and enzyme activity from both individual donors and the total pool.Gentaur guarantees performance and quality.Donor information is available. |
Immortalized Mouse Hepatocytes - SV40 |
T0101 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
Immortalized Human Hepatocytes - hTERT |
T0058 |
ABM |
1x106 cells / 1.0 ml |
Ask for price |
Immortalize Human Hepatocytes-SV40 |
CSC-I9016L |
Creative Bioarray |
One Frozen vial |
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Immortalized Hamster Hepatocytes (SV40) |
T0072 |
ABM |
1x10^6 cells / 1.0 ml |
Ask for price |
Immortalized Mouse Hepatocytes-SV40 |
CSC-I2205Z |
Creative Bioarray |
One Frozen vial |
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Immortalized Monkey Hepatocytes-SV40 |
T0073 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
Immortalized Sprague Dawley Rats Hepatocytes |
ABI-TC047D |
AcceGen |
1 vial |
Ask for price |
Description: Sprague Dawley rats hepatocytes conditionally Immortalised with temperature sensitive SV40 large T antigen. |
Human Cancer PrimaCell10: Liver Tumor Cells Growth Medium |
9-46033 |
CHI Scientific |
5 x 100 ml |
Ask for price |
Human Hepatocytes-Single Donor, Male |
ABC-TC3649 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur's hepatocytes for suspension assays (HP1 Heps-s - single donor, male) provide an economic source of characterized human hepatocytes for use in short-term suspension assays to evaluate the metabolism of drug candidates. Gentaur's hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available. |
Cryo Dog (Beagle) Hepatocytes, Plateable |
LODBCP01 |
Westburg |
each |
EUR 1002.34 |
Cryo Monkey (Cyno) Hepatocytes, Plateable |
LOCYCP01 |
Westburg |
each |
EUR 965.52 |
Cryo Dog (Beagle) Hepatocytes, Suspension |
LODBCS01 |
Westburg |
each |
EUR 390.63 |
Human Hepatocytes-Single Donor, Female |
ABC-TC3647 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur's hepatocytes for suspension assays (HP1 Heps-s, single donor, female) provide an economic source of characterized human hepatocytes for use in short-term suspension assays to evaluate the metabolism of drug candidates. Gentaur's hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available. |
Cryo Monkey (Cyno) Hepatocytes, Suspension |
LOCYCS01 |
Westburg |
each |
EUR 517.8 |
HighQC™ Mouse Hepatocytes Stem Cell |
ABC-SC0041 |
AcceGen |
1 vial |
Ask for price |
Description: Mouse Hepatocyte Stem Cells; Frozen Vial and Plated cells are available. Cells are only guaranteed with purchase of Gentaur Media and Gentaur Extra Cellular Matrix for appropriate cell culture, for 30 days from the date of shipment. |
Cryo Mouse (CD-1) Hepatocytes, Pltable |
LOMCCP01 |
Westburg |
each |
EUR 1102.09 |
Human Mature Hepatocytes, Non-Plateable |
ABC-TC3893 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur offers an assortment of various human and non-human hepatic derived cells. These include Hepatocytes, Total Liver Cell Population (TLC), Stellates, Progenitors and Intra-hepatic biliary epithelial cells. We offer these as cryopreserved cells for convenience. Cryopreserved cells are suitable for a variety of assays including induction, toxicity, drug metabolism and systems biology. Both adherent and suspension cells are available. Custom configurations are available upon request. |
Cryo Monkey (Rhesus) Hepatocytes, Plateable |
LORHCP01 |
Westburg |
each |
EUR 965.52 |
Cryo monkey (Rhesus) Hepatocytes, Suspension |
LORHCS01 |
Westburg |
each |
EUR 517.8 |
Rat Bone PrimaCell: Normal Osteoblasts |
2-82510 |
CHI Scientific |
1 Kit |
Ask for price |
Rat Heart PrimaCell 6: Cardiomyocytes |
2-82593 |
CHI Scientific |
1 Kit |
Ask for price |
HighQC™ Human IPSC-Derived Hepatocytes |
ABC-SC2119 |
AcceGen |
1 vial |
Ask for price |
Description: These hepatocytes overcome the functionality, reproducibility, and availability limitations of existing primary cell and tumor-derived cell models by providing the following:Relevant hepatic functionality and phenotypic stabilityExpression of clinical targets that are relevant for liver and metabolic diseaseCompatibility with a wide range of 3D and microphysiological systemsQuality-controlled manufacturing and batch reproducibilityReady supply in a cryopreserved format |
Hepatocytes of male Wistar rat in monolayer, in 6 well-plate |
LORIF06-EU |
Westburg |
each |
Ask for price |
Hepatocytes of male Wistar rat in monolayer, in 12 well-plate |
LORIF12-EU |
Westburg |
each |
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Hepatocytes of male Wistar rat in monolayer, in 24 well-plate |
LORIF24-EU |
Westburg |
each |
Ask for price |
Hepatocytes of male Wistar rat in monolayer, in 48 well-plate |
LORIF48-EU |
Westburg |
each |
Ask for price |
Hepatocytes of male Wistar rat in monolayer, in 96 well-plate |
LORIF96-EU |
Westburg |
each |
Ask for price |
MCHT50 - Cryo Human Hepatocytes thawing media |
LOMCHT50 |
Westburg |
each |
EUR 102.95 |
Immortalized Monkey Hepatocytes-SV40 T+t |
T0074 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
C57BL/6 Mouse Hepatocytes Suspension-Adult |
ABC-TC3192 |
AcceGen |
1 vial |
Ask for price |
|
Description: C57BL/6 Mouse Hepatocytes Suspension - Adult |
Immortalized Hamster Hepatocytes-SV40 T+t |
T0071 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
Male Beagle dog HEPATOCYTES , 10 million cells |
LODBFS10-EU |
Westburg |
each |
Ask for price |
Human Hepatocytes-Single Donor, Male; Inducible |
ABC-TC3650 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur's inducible hepatocytes (HP1 Heps-i, single donor, male) can be plated and used in long-term drug induction studies or metabolic studies where long-term incubations are required. Gentaur's cryopreserved primary human hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is tested for plating and provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available. |
Immortalized Mouse Hepatocytes-Conditionally (ImHep) |
T0099 |
ABM |
1x106 cells / 1.0 ml |
EUR 3950 |
Human Hepatocytes-Single Donor, Female; Inducible |
ABC-TC3648 |
AcceGen |
1 vial |
Ask for price |
|
Description: Gentaur's inducible hepatocytes (HP1 Heps-i, single donor, female) can be plated and used in long-term drug induction studies or metabolic studies where long-term incubations are required. Gentaur's cryopreserved primary human hepatocytes are a functional, convenient and economical solution for your drug testing and basic research needs. Each lot is tested for plating and provided with extensive characterization of metabolic functionality to ensure that you receive an ideal model for drug testing, drug development or other metabolic studies. Gentaur's human hepatocytes eliminate the need and expense of obtaining liver tissue, and conducting multiple cell isolations; and Gentaur also provides hepatocyte-tested culture reagents and instructions necessary for thawing and culturing your hepatocytes.Cell Features:Suitable for induction assays or metabolic characterization assays in plated or suspension cultures. Plated cultures are excellent for long-term intrinsic clearance assays.Hepatocytes are cryopreserved ONCE immediately after isolation and purification.Characterization of viability and enzyme activity, including fold-induction of CYP1A2, CYP2B6 and CYP3A4 for HP1 Heps-i.Gentaur guarantees performance and quality.Donor demographics and medical information are available. |
Immortalized Human Hepatocytes - HPVE6/E7 and Myc |
T0057 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 350 |
Hepatocytes of male Sprague Dawley rat in monolayer, in 6 well plate |
LORSF06-EU |
Westburg |
each |
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Hepatocytes of male Sprague Dawley rat in monolayer, in 12 well plate |
LORSF12-EU |
Westburg |
each |
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Hepatocytes of male Sprague Dawley rat in monolayer, in 24 well plate |
LORSF24-EU |
Westburg |
each |
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Hepatocytes of male Sprague Dawley rat in monolayer, in 48 well plate |
LORSF48-EU |
Westburg |
each |
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Hepatocytes of male Sprague Dawley rat in monolayer, in 96 well plate |
LORSF96-EU |
Westburg |
each |
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Hepatocytes of male Sprague Dawley rat in suspension , 10 million cells |
LORSFS10-EU |
Westburg |
each |
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Human hepatocytes in suspension , 10 million cells |
LOHUFS10-EU |
Westburg |
each |
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Rat Bone Marrow PrimaCell: Hematopoietic Cells |
2-82590 |
CHI Scientific |
1 Kit |
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Rat Thyroid PrimaCell: Thyroid Epithelial Cells |
2-82598 |
CHI Scientific |
1 Kit |
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Rat Pancreas PrimaCell: Normal Pancreatic Islets |
2-82578 |
CHI Scientific |
1 Kit |
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Male Cynomolgus monkey HEPATOCYTES , 10 million cells |
LOCYFS10-EU |
Westburg |
each |
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Rat Bone Joint PrimaCell: Normal Synovial Cells |
2-82509 |
CHI Scientific |
1 Kit |
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Rat Kidney PrimaCell 6: Proximal Tubular Cells |
2-82594 |
CHI Scientific |
1 Kit |
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Rat Cartilage PrimaCell: Normal Articular Cartilage |
2-82523 |
CHI Scientific |
1 Kit |
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Immortalized Human Hepatocytes - HPV E6/E7, hTERT & MycT58A |
T0063 |
ABM |
1x10^6 cells / 1.0 ml |
EUR 350 |
Rat Glomerular PrimaCell: Glomerular Endothelial Cells |
2-82592 |
CHI Scientific |
1 Kit |
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Rat Bone PrimaCell: Normal Osteoblasts Growth Medium |
9-25010 |
CHI Scientific |
5 x 100 ml |
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Rat Aorta PrimaCell: Normal Aortic Endothelial Cells |
2-82504 |
CHI Scientific |
1 Kit |
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Amongst the liver cell varieties, decreased cell affiliation and the absence of lipid peroxidation resulted in low cytotoxicity in LSECs and hepatocytes. Utilizing further GO samples with totally different lateral sizes, floor functionalities, or thickness, we additional confirmed the differential cytotoxic results in liver cells and the key position of GO lateral measurement in KUP5 pyroptosis by correlation research. These findings delineated the GO results on mobile uptake and cell loss of life pathways in liver cells, and supply useful data to additional consider GO results on the liver for biomedical functions.